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In 1448 Seraphina married Alessandro Sforza, Lord of Pesaro. Ten years afterwards her husband gave himself up to a dissolute life, and ill-treated her. He forced her to enter the convent of Poor Clares at Pesaro.
In 1475 Seraphina was elected abbess of the monastery at Pesaro, where she died in 1478. Her body, exhumed some years after her death, was found incorrupt, and is preserved in Pesaro Cathedral.